human foreskin fibroblasts  (ATCC)

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of early damage and stress-response genes ( a ) FDXR , ( b ) GADD45A , ( c ) SESN1 , and ( d ) GDF15 in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ordinary one-way ANOVA followed by Dunnett’s post hoc test, comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of DNA repair and antioxidant genes ( a ) DDB2 , ( b ) RNF8 , and ( c ) SOD1 in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, *** p < 0.001, by ordinary one-way ANOVA followed by Dunnett’s post hoc test comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of cell-cycle regulation and proliferation genes ( a ) CDKN1A , ( b ) CDKN2A , ( c ) MKI67 , ( d ) H2AFX , and ( e ) VEGFA in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ordinary one-way ANOVA followed by Dunnett’s post hoc test, comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of inflammatory response genes ( a ) IL-6 and ( b ) TNFAIP3 in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, **** p < 0.0001 by ordinary one-way ANOVA followed by Dunnett’s post hoc test, comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: bioRxiv

Article Title: Fibroblasts regulate lymphatic barrier functions in a tissue dependent manner

doi: 10.1101/2025.04.17.649442

Figure Lengend Snippet: (A) Schematic diagram of transwell secretome experiment. ( B) TEER measurements of hLEC monolayers after 48 hours of treatment with dermal fibroblast secretomes with [NHDF(G)] and without growth factors [NHDF(B)]. ( C) Representative immunofluorescence images of VE-cadherin, claudin-5, and ZO-1 on hLECs following treatment with NHDF secretomes. (D) Quantification of VE-cadherin, claudin-5, and ZO-1 expression levels using ImageJ (FIJI). (E) Quantification of lymphatic markers LYVE-1 and PROX1 expression in hLEC monolayers treated with NHDF secretomes. (F) FITC–Dextran (4 kDa) transport assay across hLECs after NHDF secretome treatment. Data are presented as mean ± SEM from n = 6 independent experiments. Statistical analysis was performed using an unpaired two-tailed t -test; p > 0.05 = not significant (ns), p ≤ 0.0001 = extremely significant (****). For the permeability (transport) assay ( n = 3, representative), statistical significance was determined by two-way ANOVA followed by appropriate post hoc test for multiple comparisons.

Article Snippet: Normal human primary dermal fibroblasts (NHDF) were cultured in two conditions: (1) NHDF(G) (PromoCell, C-12302) in Fibroblast Growth Medium-2 (PromoCell, C-23120) containing 2% FBS, 1 ng/mL fibroblast growth factor, and 5 μg/mL insulin, or (2) NHDF(B) in fibroblast basal medium without supplements.

Techniques: Immunofluorescence, Expressing, Transport Assay, Two Tailed Test, Permeability

Journal: bioRxiv

Article Title: Fibroblasts regulate lymphatic barrier functions in a tissue dependent manner

doi: 10.1101/2025.04.17.649442

Figure Lengend Snippet: (A) Schematic diagram of transwell co-culture experiment. (B ) Schematic diagram of cells seedings and co-culture. (C) TEER measurements of hLEC monolayers after 48 hours of co-culture with dermal fibroblast with [NHDF(G)] and without growth factors [NHDF(B)]. (D) Representative immunofluorescence images of VE-cadherin, and ZO-1 on hLECs following co-culture with NHDFs. (E) Quantification of VE-cadherin and ZO-1 expression levels using ImageJ (FIJI). (F) FITC–Dextran (4 kDa) transport assay across hLECs after co-culture with NHDFs. Data are presented as mean ± SEM from n = 6 independent experiments. Statistical analysis was performed using an unpaired two-tailed t -test; p > 0.05 = not significant (ns), p ≤ 0.0001 = extremely significant (****). For the permeability (transport) assay ( n = 5, representative), statistical significance was determined by two-way ANOVA followed by appropriate post hoc test for multiple comparisons.

Article Snippet: Normal human primary dermal fibroblasts (NHDF) were cultured in two conditions: (1) NHDF(G) (PromoCell, C-12302) in Fibroblast Growth Medium-2 (PromoCell, C-23120) containing 2% FBS, 1 ng/mL fibroblast growth factor, and 5 μg/mL insulin, or (2) NHDF(B) in fibroblast basal medium without supplements.

Techniques: Co-Culture Assay, Immunofluorescence, Expressing, Transport Assay, Two Tailed Test, Permeability

Journal: bioRxiv

Article Title: Fibroblasts regulate lymphatic barrier functions in a tissue dependent manner

doi: 10.1101/2025.04.17.649442

Figure Lengend Snippet: (A) TEER measurements of hLEC monolayers after 4 hours of thrombin treatment followed by 48 hours of co-culture with dermal fibroblast (NHDF(G) ( n = 3 ). (B) Total cell counts ( n = 6 ). (C) FITC–Dextran (4 kDa) transport assay across hLECs after 4 hours of thrombin stimulation and/or NHDF co-culture for 48 hours. (D) Representative immunofluorescence images of ZO-1 and VE-cadherin on hLECs with and without thrombin treatment. Data are presented as mean ± SEM from n = 3-6 independent experiments. Statistical analysis was performed using an unpaired two-tailed t -test; p > 0.05 = not significant (ns), p ≤ 0.0001 = extremely significant (****). For the transport assay, statistical significance was determined by two-way ANOVA followed by appropriate post hoc test for multiple comparisons.

Article Snippet: Normal human primary dermal fibroblasts (NHDF) were cultured in two conditions: (1) NHDF(G) (PromoCell, C-12302) in Fibroblast Growth Medium-2 (PromoCell, C-23120) containing 2% FBS, 1 ng/mL fibroblast growth factor, and 5 μg/mL insulin, or (2) NHDF(B) in fibroblast basal medium without supplements.

Techniques: Co-Culture Assay, Transport Assay, Immunofluorescence, Two Tailed Test